Sars/CoV2 genome

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TimGDixon
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Joined: Fri Jun 26, 2020 4:36 am

Sars/CoV2 genome

Post by TimGDixon »

This is most interesting to us. There are many corona viri but only the Betacoronaviruses can infect humans. There are 29,903 nucleotides(genes) in Sars/CoV2. Out of those 29,903 only the genes you see sequenced above are involved. Of those 4 are functional and the rest are accessory. You have heard them mention the "spike" protein, its actually a surface glycoprotein that has little spikes (hence the term spike protein) but from the spike protein comes the envelope protein followed by a membrane protein and finally a nucleocaspid protein. That is the function virus and the host is now infected. But what about the accessory genes; what do they do? They are known as open relay frame and are identifed as ORF1a, 1b, 3, 6, 7a, 7b, 8, 9b.

Notice that both Sars/CoV and Sars/CoV2 have ORF8 (a,b) accesory genes, yet OC43, HKU1, do not, but MERS does. This is what causes SARS (severe acute respiratory syndrome, ARDS (acute respiratory distress syndrome) and MERS (middle east respiratory syndrome). All three affect the respiratory system and here is what we believe occurs. Only ORF8, a gene of viral origin can activate the human gene NLRP3. NLRP3 is the mediator of SARS/ARDS/MERS. NLRP3 secretes two interleukins (inflammatory molecules) known as IL-1 and IL-18. They are what we call an "inflammasome". The NLRP3 secreted inflammasome invades the epithelium of the lung and the cytokine storm begins.

We are not virologists, but if we were, our target would be to silence ORF8, or the entire family (ORF1-10) - if you could successfully silence ORF8 there would be no activation (called acetylation) of NLRP3. Everyone would still get infected, and no one would have symptoms. A completely harmless virus.

CoV_a-b.jpg
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Howzitgoing
Posts: 29
Joined: Mon Jul 13, 2020 8:37 am

Re: Sars/CoV2 genome

Post by Howzitgoing »

Tim, the graphics, diagrams, pics are a huge help in attempting to understand just how stealthy the virus is (Lessons for SEAL teams in adaptability? Only half-joking.). Anyway, fascinating article - not just images - but new knowledge about how the virus attacks, adapts, survives. Let's hear it for cryo-electron microscopes.

... thousands of coronaviruses packed in the ice like jellybeans in a jar. They were beautifully intact, allowing him to inspect details on the viruses that measured less than a millionth of an inch.

A cryo-electron tomography image of SARS-CoV-2 viruses, in gray, with a computer reconstruction of one virus.
Coronavirus Unveiled.jpg
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Spike proteins ... soon became clear that the name is a misnomer. The spike protein is not sharp, narrow or rigid.

Each spike protein snaps together with two others, forming a structure that has a tulip-like shape. A long stem anchors the proteins to the virus, and their top looks like a three-part flower.

The spikes can be attacked by antibodies, the powerful soldiers of our immune system. To hide, they create a shield out of sugar.


https://www.nytimes.com/interactive/202 ... e=Homepage
The Coronavirus Unveiled
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